The 15-mer sequence, GGTCCT+T+A+CTTGGTG, was synthesized with either Affinity Plus or other locked nucleic acid modifications incorporated at the +T, +A, and +C sites. The percentages of duplex melted base pairs are plotted as a function of temperature. Sequences containing Affinity Plus show identical annealing properties to locked nucleic acid sequences from another manufacturer. Shorter amplicons of 80−100 bp are typically recommended for digital PCR (dPCR) or for applications with samples containing shortened fragments (such as FFPE or small circulatingįigure 1.
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